Weiyel Reference Materials
Subculture | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
Growth conditions | 37 ℃, aerobic, 18-24h |
Storage conditions | -80 ℃, cold storage away from light; Dry ice transportation |
Safety level | 1 |
Application | Sensitive test; Antibacterial test; Food testing; Cosmetic testing; Culture control; Quality control strain; API verification bacteria, GB 4789.38-2012 Escherichia coli count positive control strain. |
Sharing mode | Public welfare sharing |