Weiyel Reference Materials
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| Subculture | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
| Subculturing | Before use, the reference product is placed at room temperature for 30min, fully melted, shaken and mixed, used after nucleic acid extraction, nucleic acid extraction or purification reagents need to be provided by oneself. |
| Growth conditions | Culture temperature 37 ℃; Culture time days; 18-24h; Aerobic gas environment; |
| Storage conditions | -80 ℃, kept away from light |
| Safety level | 1 |
| Application | Accurate and rapid identification of pathogenic bacteria can effectively improve the pertinence and effectiveness of clinical treatment. This product is an E. coli nucleic acid detection reagent evaluation with heat inactivation strong positive reference for early diagnosis and quality control of infection. |
| Sharing mode | Public welfare sharing |
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