Weiyel Reference Materials
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| Subculture | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
| Subculturing | Before use, the nucleic acid reference is dissolved on ice, slightly mixed and briefly centrifuged and placed on ice for later use. |
| Growth conditions | 30 ℃,18-24h, aerobic |
| Storage conditions | -20 ℃, cold storage |
| Safety level | 1 |
| Isolation | Manganese oxide crusts from pipe |
| Application | It is used for the confirmation and evaluation of relevant measurement methods in the experimental analysis of Pseudomonas putida nucleic acid detection, and the quality control of the analysis process. |
| Sharing mode | Public welfare sharing |
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