Salmonella enterica nucleic acid detection standard|BNCC362297 |BNCC

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Salmonella enterica nucleic acid detection standard

Subculture	Tryptone soybean medium (English name: TSA/TSB): tryptone 17.0g, soybean peptone 3.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 2.5g, glucose 2.5g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.3±0.2. Sterilization at 121 ℃ for 15min.
Subculturing	Before use, it should be taken out of the low temperature environment and balanced to room temperature. Please centrifuge (12000rmp,60s) before opening the lid; After opening the lid, add 100 μL of enzyme-free ddH2O to obtain 100 μL of nucleic acid standard solution; The cover is fully shaken and mixed, and then temporarily centrifuged and placed on ice for later use.
Growth conditions	37 ℃,22h, aerobic
Storage conditions	-80 ℃
Safety level	2
Isolation	Food Poisoning; Feces, Human, 1911
Application	It is suitable for qualitative and quantitative research and analysis of Salmonella enteritidis, verification and evaluation of measurement methods and laboratory quality control.
Sharing mode	Public welfare sharing