Vibrio parahaemolyticus nucleic acid detection standard|BNCC362411 |BNCC

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Vibrio parahaemolyticus nucleic acid detection standard

Subculture	3.5% sodium chloride agar medium (English name: NA-2): beef paste 3g, peptone 10.0g,NaCl 35.0g, agar 20.0g (without liquid medium),pH 7.2~7.4. Sterilization at 121 ℃ for 15min.
Subculturing	Before use, it should be taken out of the low temperature environment and balanced to room temperature. Please centrifuge (12000rmp,60s) before opening the lid; After opening the lid, add 100 μL of enzyme-free ddH2O to obtain 100 μL of nucleic acid standard solution; The cover is fully shaken and mixed, and then temporarily centrifuged and placed on ice for later use.
Growth conditions	37 ℃;18-24h; Aerobic;
Storage conditions	-20 ℃
Safety level	2
Application	It is suitable for the assessment and verification of laboratory nucleic acid detection capabilities (sensitivity test and specificity test); it can carry out indoor quality control on the relevant laboratories of Vibrio parahaemolyticus nucleic acid detection, and effectively monitor the status of the instrument, personnel operation, and the effectiveness of the kit, etc. Ensure the accuracy and reliability of the test results, but it is not used for reagent evaluation and negative-positive result judgment.
Sharing mode	Public welfare sharing