Bordetella pertussis nucleic acid detection standard|BNCC362461 |BNCC

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Bordetella pertussis nucleic acid detection standard

Subculture	Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculturing	Before use, it should be taken out of the low temperature environment and balanced to room temperature. Please centrifuge (12000rmp,60s) before opening the lid; After opening the lid, add 100 μL of enzyme-free ddH2O to obtain 100 μL of nucleic acid standard solution; The cover is fully shaken and mixed, and then temporarily centrifuged and placed on ice for later use.
Growth conditions	37 ℃;2-3 days; Aerobic;
Storage conditions	null
Safety level	2
Application	It is suitable for qualitative and quantitative research and analysis of Bordetella pertussis, verification and evaluation of measurement methods and laboratory quality control.
Sharing mode	Public welfare sharing