Pseudomonas fluorescens DNA standard|BNCC363245 |BNCC

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Pseudomonas fluorescens DNA standard

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	When this standard is used, it is moved to room temperature to be completely dissolved, shaken evenly, and used after instantaneous centrifugation to avoid repeated freezing and thawing as much as possible.
Growth conditions	30°C;18-24h; Aerobic;
Storage conditions	-80 ℃
Safety level	1
Application	The DNA standard of Pseudomonas fluorescens can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. through fluorescence quantitative pcr combined with digital PCR determination.
Sharing mode	Public welfare sharing