Weiyel Reference Materials
Subculture | Columbia agar chocolate plate (CA-C): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 80-90 ℃, add 5% sterile defibrated sheep blood, shake well and pour the plate to cool for later use. |
Subculturing | When this standard is used, it is moved to room temperature to be completely dissolved, shaken evenly, and used after instantaneous centrifugation to avoid repeated freezing and thawing as much as possible. |
Growth conditions | 37 ℃;24-48h;5% CO2; |
Storage conditions | 2-8 ℃ |
Safety level | 2 |
Morphology | The colony diameter is 1-2mm, round, with neat edges, opaque, gray-white on the front, gray-white on the back, convex in the middle, smooth surface, bright surface, moist texture, easy to stir up, G-(red), bacilli, purity: pure |
Application | It is suitable for qualitative and quantitative research and analysis of Haemophilus parainfluenzae, verification of measurement methods, development and evaluation of nucleic acid detection kits, and laboratory quality control. |
Sharing mode | Public welfare sharing |