Weiyel Reference Materials
Subculture | Columbia blood plate (CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use. |
Subculturing | When this standard is used, it is moved to room temperature to be completely dissolved, shaken evenly, and used after instantaneous centrifugation to avoid repeated freezing and thawing as much as possible. |
Growth conditions | 37 ℃;8-10 days; Anaerobic |
Storage conditions | -80 ℃ |
Safety level | 2 |
Morphology | The colony diameter is 1-2mm, round, with neat edges, opaque, black on the front, convex in the middle, smooth surface, bright surface, moist texture, easy to stir up, G-(red), coccolidia, purity: pure |
Isolation | Human gingival sulcus |
Application | It is suitable for qualitative and quantitative research and analysis of Porphyromonas gingivalis, verification of measurement methods, development and evaluation of relevant nucleic acid detection kits, and laboratory quality control. |
Sharing mode | Public welfare sharing |