Weiyel Reference Materials
Subculture | Brain heart extract medium (English name: BHIA/BHIB): brain extract 5.0g, heart extract 12.5g, peptone 5.0g, tryptone 10.0g, sodium chloride 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 20.0g (not included in liquid medium),pH 7.4±0.2. Sterilization at 121 ℃ for 15min. |
Subculturing | When this standard is used, it is moved to room temperature to be completely dissolved, shaken evenly, and used after instantaneous centrifugation to avoid repeated freezing and thawing as much as possible. |
Growth conditions | Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic gas environment; |
Storage conditions | -80 ℃ |
Safety level | 1 |
Application | It is suitable for qualitative and quantitative research and analysis of Enterococcus faecalis, verification and evaluation of measurement methods, and laboratory quality control. |
Sharing mode | Public welfare sharing |