Lactobacillus gasseri DNA lab quality control|BNCC369825 |BNCC

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Lactobacillus gasseri DNA lab quality control

Subculture	Peptone: 10.0, beef extract powder: 8.0, yeast powder: 4.0, glucose: 20.0, magnesium sulfate: 0.2, sodium acetate: 5.0, diammonium citrate: 2.0, dipotassium hydrogen phosphate: 2.0, manganese sulfate: 0.04, tween 80:1.0, agar: 14.0,pH:5.7±0.2(25 ℃)
Subculturing	Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl.
Growth conditions	37 ℃;24-48h;5% CO2
Storage conditions	-80 ℃
Safety level	1
Isolation	human intestine
Application	The quality control is developed by Henan engineering center of industrial microbial strain, which is the inactivated bacterial suspension of Lactobacillus galactosii, and can be used for method establishment, work standard assignment, method confirmation and evaluation, product quality control, etc.
Sharing mode	Public welfare sharing