Rhodopseudomonas palustris DNA standard|BNCC371975 |BNCC

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Rhodopseudomonas palustris DNA standard

Subculture	Ammonium chloride: 1.0, potassium dihydrogen phosphate: 0.5, magnesium chloride: 0.5, calcium chloride: 0.1, sodium chloride: 1.0, sodium acetate: 2.0, sodium succinate: 1.0, yeast extract powder: 0.5, peptone: 0.5, ferrous chloride: 1.8mg, cobalt chloride: 0.25mg, nickel chloride: 0.01mg, copper chloride: 0.01mg, manganese chloride: 0.07mg, zinc chloride: 0.1mg, boric acid: 0.5mg, sodium selenite: 0.01mg, sodium molybdate: sodium molybdate: 0.01g, biotin: 0.01mg, nicotinic acid: 0.035mg, calcium pantothenate: 0.01mg, thiamine hydrochloride: 0.03mg,VB12:0.005mg, pyridoxamine hydrochloride: 0.01mg, p-aminobenzoic acid: 0.02mg, agar: 13.0,pH:6.8(25 ℃)
Subculturing	When using this standard, it is moved to room temperature (20~25 ℃) and then thawed, shaken evenly, centrifuged briefly, and placed on ice in a low temperature environment for temporary storage for use, trying to avoid repeated freezing and thawing.
Growth conditions	30 ℃,3-4 days, anaerobic light
Storage conditions	-80 ℃
Safety level	0
Application	The DNA standard of Rhodopseudomonas palustris, which can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. by fluorescence quantitative pcr combined with digital PCR.
Sharing mode	Public welfare sharing