Water quality fecal coliform standard quality control sample (Membrane membrane method)|BNCC360398 |BNCC

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Water quality fecal coliform standard quality control sample (Membrane membrane method)

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Samples require a total of 100mL of sterile water or sterile saline to dissolve. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biological safety cabinet, 3~4mL of sterile water is immediately added, dissolved and sucked out into a sterile bottle, the remaining sterile water is repeatedly washed into the inner wall of the Xilin bottle, and all of them are transferred to the above sterile bottle, which is the standard quality control sample, equivalent to 100mL of water sample to be tested. It is suggested that the filtration volume is 25~65mL, and the number of fecal coliform colonies is 20~60, and the total number is less than 200.
Growth conditions	Culture temperature 37 ℃; Culture time 18~24 hours; Aerobic gas environment
Storage conditions	-20 ℃, kept away from light
Safety level	1
Application	Suitable for water quality fecal coliform determination quality control and personnel assessment (filter membrane method). Reference Standard "HJ 347.1-2018 Water Quality Determination of Fecal Coliform Bacteria Filter Method"
Sharing mode	Public welfare sharing