Quality control sample of total number of feed colonies|BNCC361566 |BNCC

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Quality control sample of total number of feed colonies

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	After taking out the quality control sample from the refrigerator at -20 ℃, placing it at room temperature for 30 min, then opening it in the biological safety cabinet, immediately adding 1.1mL of sterile water or sterile physiological saline to re-dissolve the freeze-dried quality control product, and fully mixing it to prepare a sample suspension, which is the quality control sample with a concentration of 0.6-1.2 × 10 5cfu/ml.
Growth conditions	37 ℃;18-24h; Aerobic;
Storage conditions	-20 ℃
Safety level	2
Application	GB/T 13093-2006 Determination of the total number of bacteria in feed, etc.
Sharing mode	Public welfare sharing