Weiyel Reference Materials
Subculture | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
Subculturing | Before use, the reference product is placed at room temperature for 30min, fully melted, shaken and mixed, used after nucleic acid extraction, nucleic acid extraction or purification reagents need to be provided by oneself. |
Growth conditions | Culture temperature 37 ℃;18-24 culture time hours; Aerobic gas environment; |
Storage conditions | -80 ℃ |
Safety level | 2 |
Application | Research on Reagents for Detection of Pathogen Nucleic Acid |
Sharing mode | Public welfare sharing |