Weiyel Reference Materials
Subculture | Potato Glucose Agar (English Name: PDA): Potato Boiled Solution 1.0L, Glucose 20.0g, Agar 15.0g, Natural pH. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L. |
Subculturing | This reference does not recommend repeated freezing and thawing. Before use, take it out of the refrigerator, balance to room temperature, mix well, and use it. |
Growth conditions | Culture temperature 28 ℃; Culture time 48-72 days; Aerobic gas environment; |
Storage conditions | -80 ℃ |
Safety level | 1 |
Morphology | The colony diameter is 2-4mm, round, with neat edges, opaque, white on the front, light color, convex in the middle, smooth surface, bright surface, moist texture, easy to stir up, no pigment, G + (blue-purple), cocci, purity: pure |
Application | Pathogenic nucleic acid detection kit cross-reaction, specificity test, and other nucleic acid related studies. |
Sharing mode | Public welfare sharing |