Fecal coliform positive control quality control sample (Multi-tube fermentation method)|BNCC359094 |BNCC

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Fecal coliform positive control quality control sample (Multi-tube fermentation method)

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Samples require a total of 200mL of sterile water or sterile saline to dissolve. Before use, the quality control sample is placed at room temperature for 30min, followed by biological safety Open the whole cabinet, immediately add 3~4mL of sterile water, dissolve it and suck it out into a sterile bottle, repeatedly wash the inner wall of the vials with the remaining sterile water, and turn it all around. When moved to the above sterile bottle, it is a positive quality control sample, totaling 200mL, with a concentration of 300 ~ 3000MPN/L.
Growth conditions	37°C, aerobic, 18-24h
Storage conditions	-20 ℃, kept away from light
Safety level	1
Application	It is suitable for the determination of fecal coliforms in water quality, quality control and personnel assessment (multi-tube fermentation method). Reference Standard "HJ 347.2-2018 Water Quality Determination of Fecal Coliforms Multi-tube Fermentation Method"
Sharing mode	Public welfare sharing