Weiyel Reference Materials
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| Subculture | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
| Subculturing | This reference does not recommend repeated freezing and thawing. Before use, take it out of the refrigerator, balance to room temperature, mix well, and use it. |
| Growth conditions | Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic gas environment; |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| Morphology | Colony morphology: colonies formed on DHL agar plate are light pink, large and raised, smooth and moist, mucus-like, and adjacent colonies are easy to melt into pus-like; Microscopic morphology: Gram-negative Brevibacterium, without spores, single, double or short-chain oval or rod-shaped. |
| Application | It can be used for specific analysis and cross-reaction verification of nucleic acid detection kits. |
| Sharing mode | Public welfare sharing |
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