Weiyel Reference Materials
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| Subculture | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
| Subculturing | This reference does not recommend repeated freezing and thawing. Before use, take it out of the refrigerator, balance to room temperature, mix well, and use it. |
| Growth conditions | Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic gas environment; |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| Morphology | Colony morphology: colonies formed on DHL agar plate are light pink, large and raised, smooth and moist, mucus-like, and adjacent colonies are easy to melt into pus-like; Microscopic morphology: Gram-negative Brevibacterium, without spores, single, double or short-chain oval or rod-shaped. |
| Application | It can be used for specific analysis and cross-reaction verification of nucleic acid detection kits. |
| Sharing mode | Public welfare sharing |
Filobasidiella bacillispora nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC361399
Bordetella pertussis DNA lab quality control
BNCC363480
Escherichia coli bacteriophage T1 nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC362933
Serratia marcescens DNA lab quality control product
BNCC363434
Shigella flexneri nucleic acid detection standard
BNCC362425
Escherichia bacteriophage Qbeta nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC363084