Weiyel Reference Materials
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| Subculture | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
| Subculturing | This reference does not recommend repeated freezing and thawing. Before use, take it out of the refrigerator, balance to room temperature, mix well, and use it. |
| Growth conditions | Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic gas environment; |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| Application | This reference product is developed by Henan Industrial Microbial Strain Engineering Technology Research Center. It can be used for specific analysis of nucleic acid detection kits, cross-reaction verification, measurement method evaluation and laboratory quality control. |
| Sharing mode | Public welfare sharing |
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