Bordetella pertussis nucleic acid detection standard|BNCC362325 |BNCC

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Bordetella pertussis nucleic acid detection standard

Subculture	Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculturing	When this standard is used, it is moved to room temperature (20~25 ℃) for re-melting, oscillates evenly, centrifuges briefly, and is temporarily stored for use in a low temperature environment on ice to avoid repeated freezing and thawing.
Growth conditions	37 ℃;2-3 days; Aerobic;
Storage conditions	-80 ℃
Safety level	2
Application	It is suitable for qualitative and quantitative research and analysis of Bordetella pertussis, verification and evaluation of measurement methods and laboratory quality control.
Sharing mode	Public welfare sharing