Weiyel Reference Materials
Subculture | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
Subculturing | When this standard is used, it is moved to room temperature to be completely dissolved, shaken evenly, and used after instantaneous centrifugation to avoid repeated freezing and thawing as much as possible. |
Growth conditions | 37 ℃;18-24h; Aerobic; |
Storage conditions | -80 ℃ |
Safety level | 1 |
Isolation | Fecal specimens from diarrhea cases |
Application | Enteropathogenic Escherichia coli (EPEC)DNA standard is mainly used for positive control, nucleic acid detection kit development and related research. |
Sharing mode | Public welfare sharing |