Clostridium difficile Prevot nucleic acid detection standard|BNCC362394 |BNCC

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Clostridium difficile Prevot nucleic acid detection standard

Subculture	Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculturing	Before use, it should be taken out of the low temperature environment and balanced to room temperature. Please centrifuge (12000rmp,60s) before opening the lid; After opening the lid, add 100 μL of enzyme-free ddH2O to obtain 100 μL of nucleic acid standard solution; The cover is fully shaken and mixed, and then temporarily centrifuged and placed on ice for later use.
Growth conditions	Culture temperature 37 ℃; Culture time 48 hours; Anaerobic gas environment;
Storage conditions	-20 ℃
Safety level	0
Application	It is suitable for the assessment and verification of laboratory nucleic acid detection capabilities (sensitivity test and specificity test); it can carry out indoor quality control on the relevant laboratories of Clostridium difficile nucleic acid detection, effectively monitor the status of the instrument, personnel operation, and the effectiveness of the kit, etc. Ensure the accuracy and reliability of the test results, but it is not used for reagent evaluation and negative-positive result judgment.
Sharing mode	Public welfare sharing