Weiyel Reference Materials
Subculture | Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use. |
Subculturing | When this standard is used, it is moved to room temperature to be completely dissolved, shaken evenly, and used after instantaneous centrifugation to avoid repeated freezing and thawing as much as possible. |
Growth conditions | Culture temperature 37 ℃;18-24h; Gas environment 5% CO2; |
Storage conditions | -20 ℃ |
Safety level | 2 |
Application | It is suitable for qualitative and quantitative research and analysis of group A streptococcus, verification and evaluation of measurement methods and laboratory quality control. |
Sharing mode | Public welfare sharing |