Shigella flexneri (Hrug resistance)(Heat inactivated) (Strongly positive)|BNCC362724 |BNCC

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Shigella flexneri (Hrug resistance)(Heat inactivated) (Strongly positive)

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Pick a single colony and add bacteria in a liquid triangular flask, then take 25mL of bacteria liquid and centrifuge to collect bacteria (centrifuge speed 8000r /min,30s), resuspend with 10mLPBS, and collect bacteria suspension for later use. The concentration of bacterial suspension is about 109CFU/mL, diluted to 10-6, 10-7, 10-8 gradient, take 200 μL coating count, select the colony number between 30 CFU to 300 CFU plate count;
Growth conditions	Culture temperature 37 ℃;18-24 hours; Aerobic gas environment;
Storage conditions	-80 ℃
Safety level	0
Application	Accurate and rapid identification of pathogenic bacteria can effectively improve the pertinence and effectiveness of clinical treatment. This product is a heat-inactivated strong positive reference for the evaluation of Shigella flexneri nucleic acid test for early diagnosis of infection And quality control.
Sharing mode	Public welfare sharing