Weiyel Reference Materials
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| Subculture | Yeast extract: 3.0, malt extract: 3.0, glucose: 10.0, casein peptone: 5.0,pH:6.2±0.2(25 ℃) |
| Subculturing | Inoculate YM broth medium, collect by centrifugation, resuspend in PBS, inactivate in water bath at 65 ℃ for 30min |
| Growth conditions | 37 ℃;48-72h; strictly anaerobic |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| Morphology | The colony is 0.5-1mm in diameter, round, with irregular edges, opaque, gray-white front, convex in the middle, smooth surface, dark surface, moist texture, easy to provoke, G-(red), bacilli |
| Isolation | human feces |
| Sharing mode | Public welfare sharing |
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