Weiyel Reference Materials
Subculture | Yeast extract: 3.0, malt extract: 3.0, glucose: 10.0, casein peptone: 5.0,pH:6.2±0.2(25 ℃) |
Subculturing | Inoculate YM broth medium, collect by centrifugation, resuspend in PBS, inactivate in water bath at 65 ℃ for 30min |
Growth conditions | 37 ℃;48-72h; strictly anaerobic |
Storage conditions | -80 ℃ |
Safety level | 1 |
Morphology | The colony is 0.5-1mm in diameter, round, with irregular edges, opaque, gray-white front, convex in the middle, smooth surface, dark surface, moist texture, easy to provoke, G-(red), bacilli |
Isolation | human feces |
Sharing mode | Public welfare sharing |