Weiyel Reference Materials
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| Subculture | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
| Subculturing | When this standard is used, it is moved to room temperature to be completely dissolved, shaken evenly, and used after instantaneous centrifugation to avoid repeated freezing and thawing as much as possible. |
| Growth conditions | 37 ℃,18-24h, aerobic |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| Morphology | The colony diameter is 1-2mm, round, with neat edges, opaque, gray-white on the front, smooth surface, convex in the middle, bright surface, moist texture, easy to stir up, G-(red), bacilli, purity: pure |
| Application | It is suitable for qualitative and quantitative research and analysis of Klebsiella pneumoniae, verification of measurement methods, development and evaluation of nucleic acid detection kits, and laboratory quality control. |
| Sharing mode | Public welfare sharing |
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