Bacillus subtilis spore suspension|BNCC358969 |BNCC

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Bacillus subtilis spore suspension

Subculture	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	(1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then pack and freeze-dry;
Growth conditions	30 ℃;18-24h; Aerobic;
Storage conditions	2-8 ℃
Safety level	1
Morphology	The colony diameter is 1-2mm, round, yellow on the front, light in color, convex in the middle, bright and opaque surface, neat edges, smooth surface, moist texture, G + (blue-purple), bacilli
Application	Resistance Testing Dry heat sterilization control Efficacy testing Ethylene oxide sterilization control Media testing Steam sterilization control Sterility assurance Sterilization control Pharmaceutical and Personal Care Resistance test dry heat sterilization control efficacy test ethylene oxide sterilization control medium inspection BR> sterility assurance sterilization control> drugs and personal care
Sharing mode	Public welfare sharing