Weiyel Reference Materials
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| Subculture | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
| Subculturing | When this standard sample is used, it is moved to room temperature (20~25 ℃) for re-melting, shaken evenly, centrifuged briefly, and placed on ice in a low temperature environment for temporary storage for use, so as to avoid repeated freezing and thawing. |
| Growth conditions | 37°C;18-24h; Aerobic |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| Application | This Streptococcus suis DNA standard, through fluorescence quantitative pcr combined with digital PCR, can be used for method establishment, work standard assignment, method confirmation and evaluation, product quality control, etc. |
| Sharing mode | Public welfare sharing |
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