Weiyel Reference Materials
Subculture | Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃) |
Subculturing | Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl. |
Growth conditions | 37 ℃;18-24h; aerobic; |
Storage conditions | -80 ℃ |
Safety level | 2 |
Isolation | Tissue, animal - pools of heart and liver from 4-week-old chickens |
Application | The quality control is Salmonella enteritidis inactivated bacterial suspension, which can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. by digital PCR value. |
Sharing mode | Public welfare sharing |