Escherichia coli DNA standard|BNCC363451 |BNCC

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Escherichia coli DNA standard

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	When this standard is used, it is moved to room temperature to be completely dissolved, shaken evenly, and used after instantaneous centrifugation to avoid repeated freezing and thawing as much as possible.
Growth conditions	37 ℃;18-24h; Aerobic;
Storage conditions	-80 ℃
Safety level	1
Application	This DNA standard is to extract and purify nucleic acid from ATCC25922 Escherichia coli after heat inactivation treatment. The absolute concentration of nucleic acid is determined by digital PCR, and then the nucleic acid solution is diluted to the required concentration with TE buffer solution. The standard is not infectious and has no biosafety risks. It is suitable for nucleic acid detection, specific analysis and other nucleic acid related studies of ATCC25922 Escherichia coli. It cannot be used for clinical diagnosis of diseases.
Sharing mode	Public welfare sharing