Weiyel Reference Materials
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| Subculture | Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use. |
| Subculturing | Before use, the reference product is placed at room temperature for 30min, fully melted, shaken and mixed, used after nucleic acid extraction, nucleic acid extraction or purification reagents need to be provided by oneself. |
| Growth conditions | Culture temperature 37 ℃; Culture time 24-48 hours; Gas environment 5% CO2; |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| Application | Accurate and rapid identification of pathogenic bacteria can effectively improve the pertinence and effectiveness of clinical treatment. This product uses heat inactivated strong positive reference for the evaluation of the nucleic acid detection reagent of C. diphtheria for early diagnosis and quality control of infection. |
| Sharing mode | Public welfare sharing |
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