Weiyel Reference Materials
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| Subculture | EMEM complete medium (with 2% FBS):EMEM + 2% FBS |
| Subculturing | Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl. |
| Growth conditions | Culture temperature 37 ℃;3-6 days of culture time; Gas environment 5% CO2 + 95% air; |
| Storage conditions | -80 ℃ |
| Safety level | 0 |
| Application | The quality control was developed by Henan engineering center of industrial microbial strain, and was used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. by digital PCR value. |
| Sharing mode | Public welfare sharing |
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