Weiyel Reference Materials
Subculture | Bovine brain extract powder: 4.0, bovine heart extract powder: 4.0, peptone: 5.0, casein peptone: 16.0, sodium chloride: 5.0, glucose: 2.0, disodium hydrogen phosphate: 2.5, agar: 13.5,pH:7.4±0.2(25 ℃) |
Subculturing | Inoculation of brain heart infusion agar plate medium, PBS elution, suspension collection, 65 ℃ water bath inactivation for 30min |
Growth conditions | 37 ℃;18-24h;5% CO2 |
Storage conditions | -80 ℃ |
Safety level | 1 |
Morphology | The colony is 1-2mm in diameter, round, with neat edges, opaque, gray-white on the front, gray-white on the back, convex in the middle, smooth surface, bright surface, moist texture, easy to provoke, G-(red), cocci, purity: pure |
Sharing mode | Public welfare sharing |