Weiyel Reference Materials
Subculture | EMEM complete medium (containing 2% FBS):98% EMEM + 2% FBS |
Subculturing | Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl. |
Growth conditions | Culture temperature 37 ℃; Culture time 3-5 days; Gas environment 5% CO2 + 95% air; |
Storage conditions | -80 ℃ |
Safety level | 2 |
Isolation | The strain was a pig leukocyte isolated from a blood sample collected at a slaughterhouse. |
Application | This reference was developed by the Henan engineering center of industrial microbial strain. It is an inactivated particle of porcine parvovirus NADL-2. It can be used for quality control of porcine parvovirus NADL-2, standard substance development, positive control of nucleic acid detection, nucleic acid detection research, etc. |
Sharing mode | Public welfare sharing |