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| Subculture | EMEM complete medium (with 2% FBS):EMEM + 2% FBS |
| Subculturing | Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl. |
| Growth conditions | Culture temperature 37 ℃; Gas environment 5% CO2 + 95% air; |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| Application | The quality control is developed by Henan engineering center of industrial microbial strain, which is the inactivated suspension of Coxsackie virus A6, which can be used for method establishment, working standard assignment, method confirmation and evaluation by adding stabilizer and digital PCR value. |
| Sharing mode | Public welfare sharing |
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