Shigella dysenteriae DNA lab quality control|BNCC370144 |BNCC

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Shigella dysenteriae DNA lab quality control

Subculture	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculturing	Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl.
Growth conditions	37 ℃,18-24h, aerobic
Culture properties	Gram-negative bacilli, no movement, no capsule, no spore formation, no acid. Aerobic and facultative anaerobic, and grew well on simple nutrient media. It is the causative agent of bacillary dysentery and can cause bloody diarrhea. People and other large primates are the only natural hosts. Most infections are spread from person to person, but ingestion of contaminated water or food can cause infection. Sero : A5.
Storage conditions	-80 ℃
Safety level	1
Application	The quality control is developed by Henan engineering center of industrial microbial strain, which is the inactivated bacterial suspension of Shigella dysenteriae, which can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc.
Sharing mode	Public welfare sharing