Burkholderia gladioli DNA lab quality control|BNCC370151 |BNCC

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Burkholderia gladioli DNA lab quality control

Subculture	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculturing	① Prepare a test tube and 2 plates containing 5~10mL of liquid culture medium; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then crush it with tweezers; ③ Absorb 0.5mL of liquid culture medium into a freeze-drying tube, fully dissolve it, and then return it to the liquid test tube for mixing. ④ Absorb 0.2mL of bacteria into a suspension plate and coat evenly, repeat twice to obtain two plates; ⑤ Place all the liquid test tubes and plates under the above culture conditions, and the strains can be used when they grow out.
Growth conditions	30 ℃,18-24h, aerobic
Culture properties	Gram-negative bacilli, no movement, no capsule, no spore formation, no acid. Aerobic and facultative anaerobic, and grew well on simple nutrient media. It is the causative agent of bacillary dysentery and can cause bloody diarrhea. People and other large primates are the only natural hosts. Most infections are spread from person to person, but ingestion of contaminated water or food can cause infection. Sero : A5.
Storage conditions	-80 ℃
Safety level	1
Morphology	The colony has a diameter of 1-2mm, round, neat edges, opaque, yellow front, light color, raised in the middle, smooth surface, bright surface, moist texture, easy to provoke, G-(red), bacilli
Application	The quality control is developed by Henan engineering center of industrial microbial strain, which is the inactivated bacterial suspension of Gladiolus Burkholderia, which can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. by digital PCR value.
Sharing mode	Public welfare sharing