Weiyel Reference Materials
Subculture | EMEM complete medium (containing 2% FBS):98% EMEM + 2% FBS |
Subculturing | When using this standard, it is moved to room temperature (20~25 ℃) and then thawed, shaken evenly, centrifuged briefly, and placed on ice in a low temperature environment for temporary storage for use, trying to avoid repeated freezing and thawing. |
Growth conditions | Culture temperature 37 ℃; Culture time 5 days; Gas environment 5% CO2 + 95% air; |
Storage conditions | -80 ℃ |
Safety level | 1 |
Application | The human adenovirus 5 DNA standard can be used as a real-time fluorescence quantitative PCR, digital PCR, DNA probe hybridization detection and high-throughput sequencing methods for quantitative and qualitative analysis of human adenovirus 5 measurement standards, but also can be used for HAdV typing identification and detection platform quality control and other fields. |
Sharing mode | Public welfare sharing |