Weiyel Reference Materials
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| Subculture | Peptone: 10.0, beef extract powder: 8.0, yeast powder: 4.0, glucose: 20.0, magnesium sulfate: 0.2, sodium acetate: 5.0, diammonium citrate: 2.0, dipotassium hydrogen phosphate: 2.0, manganese sulfate: 0.04, tween 80:1.0, agar: 14.0,pH:5.7±0.2(25 ℃) |
| Subculturing | When using this standard, move to room temperature to be completely dissolved, shake evenly, use after instantaneous centrifugation, try to avoid repeated freezing and thawing. |
| Growth conditions | 37 ℃;24-48h; anaerobic; |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| Application | The standard, not infectious, no biosafety risk, suitable for real-time fluorescence quantitative PCR and other methods of qualitative and quantitative determination of lactic acid bacteria nucleic acid, as well as laboratory quality control and kit development and evaluation. |
| Sharing mode | Public welfare sharing |
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