Weiyel Reference Materials
Subculture | Peptone (trypsin hydrolysis):15.0, yeast extract powder: 5.0, glucose: 5.0, sodium thioglycolate: 0.5,L-cystine: 0.5, sodium chloride: 2.5, resazurin: 0.001, agar: 0.75,pH: 7.1±0.2(25 ℃) |
Subculturing | When using this standard, it is moved to room temperature (20~25 ℃) and then thawed, shaken evenly, centrifuged briefly, and placed on ice in a low temperature environment for temporary storage for use, trying to avoid repeated freezing and thawing. |
Growth conditions | 37 ℃;3-5 days; anaerobic |
Storage conditions | -80 ℃ |
Safety level | 1 |
Application | Clostridium tetani DNA standard, by fluorescence quantitative PCR combined with digital PCR value, can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. |
Sharing mode | Public welfare sharing |