Water quality enzyme substrate method negative quality control sample|BNCC360395 |BNCC

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Water quality enzyme substrate method negative quality control sample

Subculture	MMO-MUG medium: composition (g/L): ammonium sulfate 5.0; Manganese sulfate 0.0005; Zinc sulfate 0.0005; Magnesium sulfate 0.1; Sodium chloride 10.0; Calcium chloride 0.05; Sodium sulfite 0.04; Amphotericin B 0.001; ONPG 0.5;MUG 0.075;Solanium extract 0.5;HEPES 6.9;HEPES sodium salt; Take 100ml of water sample from a 100ml sterile dilution bottle and add 2.7±0.5g of this product, shake evenly to completely dissolve it, and put it into an incubator at 36±1 ℃ for 24 hours.
Subculturing	Before use, the quality control sample is placed at room temperature for 30min, and the sample needs to be dissolved in a total of 100mL of sterile water or sterile normal saline. Then open it in the biological safety cabinet, immediately add 3~4mL of sterile water, dissolve it and suck it out into a sterile bottle, repeatedly wash the inner wall of the penile bottle with the remaining sterile water, and transfer it to the above sterile bottle, which is 100mL standard sample stock solution.
Growth conditions	37 ℃; Culture time 24-48 hours; Anaerobic gas environment
Storage conditions	-20 ℃, kept away from light
Safety level	2
Application	Reference standard: HJ 1001-2018 Enzyme Substrate Method for Determination of Total Coliforms, Fecal Coliforms and Escherichia coli in Water Quality; Negative Control for Determination of Total Coliforms, Fecal Coliforms and Escherichia coli in Water Quality (Enzyme Substrate Method).
Sharing mode	Public welfare sharing