Seawater total bacterial colony count quality control sample|BNCC363184 |BNCC

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Seawater total bacterial colony count quality control sample

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Before use, the quality control sample is placed at room temperature for 30 min, then opened in the biological safety cabinet, immediately added 5mL of sterile water or sterile physiological saline to re-dissolve the freeze-dried quality control product, and the sample suspension is obtained after being fully mixed, with a concentration of 10 3~10 5CFU/mL.
Growth conditions	Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic gas environment;
Storage conditions	-20 ℃
Safety level	0
Application	It is applicable to the detection of the total number of colonies, quality control and personnel assessment in the standard "GB 17378.7-2007 Marine Monitoring Specification Part 7: Ecological Investigation and Biological Monitoring of Offshore Pollution.
Sharing mode	Public welfare sharing