Fecal coliform standard quality control sample (Multitube fermentation method)|BNCC359289 |BNCC

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Fecal coliform standard quality control sample (Multitube fermentation method)

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Samples require a total of 200mL of sterile water or sterile saline to dissolve. Before use, the quality control sample is placed at room temperature for 30min, then opened in the biological safety cabinet, 3~4mL of sterile water is immediately added, dissolved and sucked out into a sterile bottle, the remaining sterile water is repeatedly washed into the inner wall of the Xilin bottle, and all of them are transferred to the above sterile bottle, which is the standard quality control sample, equivalent to 200mL of water sample to be tested, with a concentration of 300 ~ 3000MPN/L.
Growth conditions	Culture temperature 37 ℃; Culture time 24-48 hours; Aerobic gas environment
Storage conditions	-20 ℃, kept away from light
Safety level	1
Application	It is suitable for the determination of fecal coliforms in water quality, quality control and personnel assessment (multi-tube fermentation method). Reference Standard "HJ 347.2-2018 Water Quality Determination of Fecal Coliforms Multi-tube Fermentation Method"
Sharing mode	Public welfare sharing