Lactobacillus frivolum nucleic acid reference material (Heat inactivated) (Strongly positive)|BNCC360609 |BNCC

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Lactobacillus frivolum nucleic acid reference material (Heat inactivated) (Strongly positive)

Subculture	MRS medium (English name: MRS): peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L. pH 5.7±0.2. Sterilization at 121 ℃ for 15min.
Subculturing	Before use, balance the reference at room temperature for 30min and thaw. Equilibrium to room temperature, vortex oscillation and mixing, according to the nucleic acid extraction reagent instructions to extract nucleic acid after use, nucleic acid extraction or purification reagents need to be self-provided.
Storage conditions	-80 ℃, kept away from light
Safety level	2
Application	This product is a heat-inactivated strong positive reference for the evaluation of Lactobacillus curl nucleic acid detection reagent, which can be used for method establishment, work standard assignment, method confirmation and evaluation, product quality control, etc.
Sharing mode	Public welfare sharing