Weiyel Reference Materials
Subculture | Columbia agar chocolate plate (CA-C): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 80-90 ℃, add 5% sterile defibrated sheep blood, shake well and pour the plate to cool for later use. |
Subculturing | Take it out of the refrigerator, and after it is completely melted, the vortex oscillates and mixes well, and it can be used. Note: The essential control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be provided by oneself, and repeated freezing and thawing should be avoided before extraction. The minimum sampling amount is 200 μL. |
Growth conditions | 37 ℃,18-24h,5% CO2 + 95% air |
Storage conditions | -80 ℃ |
Safety level | 2 |
Morphology | Size: 2-4mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white uplift: middle convex surface: bright and smooth texture: moist, neat edges, off-white front, dark color, smooth surface, bright surface, moist texture, easy to pick up, no pigment, G-(red), bacilli, purity: pure |
Isolation | Throat of young adult with respiratory infection |
Application | The essential control product is a non-fixed value quality control product, which is only used for precision testing in related laboratories. It can be used for indoor quality control of Haemophilus influenzae nucleic acid testing reagent manufacturers and related laboratories for Haemophilus influenzae nucleic acid testing. Effectively monitor the status of the instrument, personnel operation, effectiveness of the kit, etc., to ensure the accuracy and reliability of the test results, but it is not used for reagent evaluation and negative-positive result judgment. |
Sharing mode | Public welfare sharing |