Weiyel Reference Materials
Subculture | Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
Subculturing | Before use, the nucleic acid reference is dissolved on ice, slightly mixed and briefly centrifuged and placed on ice for later use. |
Growth conditions | 37 ℃;18-24h; Aerobic; |
Storage conditions | -80 ℃ |
Safety level | 1 |
Application | It is suitable for confirmation, evaluation and quality control of relevant measurement methods in the experimental analysis of Yersinia enterocolitica nucleic acid detection. |
Sharing mode | Public welfare sharing |