Serratia marcescens nucleic acid reference (Heat inactivated) (Strongly positive)|BNCC361551 |BNCC

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Serratia marcescens nucleic acid reference (Heat inactivated) (Strongly positive)

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Pick a single colony and add bacteria in a liquid triangular flask, then take 25mL of bacteria liquid and centrifuge to collect bacteria (centrifuge speed 8000r /min,30s), resuspend with 10mLPBS, and collect bacteria suspension for later use. The concentration of bacterial suspension is about 109CFU/mL, diluted to 10-6, 10-7, 10-8 gradient, take 200 μL coating count, select the colony number between 30 CFU to 300 CFU plate count;
Growth conditions	Culture temperature 30 ℃;18-24 hours; Aerobic gas environment;
Storage conditions	-80 ℃
Safety level	0
Application	Accurate and rapid identification of pathogenic bacteria can effectively improve the pertinence and effectiveness of clinical treatment. This product uses heat inactivated strong positive reference for the evaluation of Serratia mucilagi nucleic acid detection reagent, in order to be used for early diagnosis of infection And quality control.
Sharing mode	Public welfare sharing