Weiyel Reference Materials
Subculture | Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use. |
Subculturing | When using essential control products, move to room temperature to be completely dissolved, shake evenly, use after instantaneous centrifugation, and try to avoid repeated freezing and thawing. |
Growth conditions | 37 ℃,18-24h,5% CO2 + 95% air |
Storage conditions | -20 ℃ |
Safety level | 2 |
Application | It is used for the confirmation and evaluation of relevant measurement methods in the experimental analysis of Streptococcus pneumoniae nucleic acid detection, and the quality control of the analysis process. |
Sharing mode | Public welfare sharing |