Weiyel Reference Materials
Subculture | 90%EMEM+10%FBS |
Subculturing | Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl. |
Growth conditions | Culture temperature 37 ℃; Culture time 2-7 days; Gas environment 5% CO2 + 95% air; |
Storage conditions | -80 ℃ |
Safety level | 2 |
Isolation | This strain was isolated from the urine of a male patient with congenital cytomegalovirus inclusion disease. |
Application | The quality control is developed by Henan engineering center of industrial microbial strain, which is the inactivated suspension of human herpes virus 4 (EB virus), which can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. |
Sharing mode | Public welfare sharing |