Bacillus subtilis subsp nucleic acid reference (Heat inactivated) (Strongly positive)|BNCC361581 |BNCC

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Bacillus subtilis subsp nucleic acid reference (Heat inactivated) (Strongly positive)

Subculture	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculturing	Before use, the reference product is placed at room temperature for 30min, fully melted, shaken and mixed, used after nucleic acid extraction, nucleic acid extraction or purification reagents need to be provided by oneself.
Growth conditions	Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic gas environment;
Storage conditions	-80 ℃
Safety level	1
Application	This product is a Bacillus subtilis nucleic acid reference (heat inactivated) (strong positive), which can be used for specific analysis of nucleic acid detection kits, cross-reaction verification, measurement method evaluation and laboratory quality control.
Sharing mode	Public welfare sharing